Getting started .... using C/C++

In this example, we are going to open an SBOL file containing the expression casette for a single protein: [http://sbolstandard.org/examples/example_minimal.sbol.rdf]

We are going to use libSBOL to extract the sequence of the full construct. We will then piece together a new construct which replaces the ribosomal binding site between promoter and protein-coding sequence. We then save the new construct in a new SBOL record.

Install dependencies

You will need C/C++ (v. 199.9.3) and libSBOL.

Detailed installation instructions can be found here. With basic development tools installed, the following should work:

insert installation command(s)

Import and use SBOL files

  1. Load an SBOL file
  2. Extract the full DNA sequence from SBOL
  3. List the sub-components of a DNA construct
  4. Extract the sequence and annotation of a sub-component

Export SBOL

  1. Create a new construct from two old and one new sub-components
  2. Export the new Component (everything)
  3. Export the new Component (only what has changed)